| Name | Time | Year | |
| Kristen Stuart | 24.20 | 2003 | |
| Laurel Seiter | 24.34hc | 1981 | |
| Jada Newkirk | 24.35 | 2018 | |
| Jacqui Dent | 24.54 | 1991 | |
| LaShauna Porter | 24.66 | 2018 | |
| Christina Soria | 24.90 | 1998 | |
| Emma Yudelevitch | 24.90 | 2017 | |
| Marshairi Dunn | 25.00 | 1991 | |
| Desirie Rippy | 25.17 | 1994 | |
| Binti Allen | 25.32 | 1991 | |
| Emma Yudelevitch | 25.40 | 2016 | |
| Onyekachi Nwabueze | 25.63 | 2016 | |
| La Juan Washington | 25.84 | 1980 |